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Pomer polynenasýtených mastných kyselín n-6 a n-3 vo vzťahu k riziku srdcovo-cievnych ochorení
Gregorová, Lívia
The aim of the bachelor's thesis was to create literature research using literature sources to clarify the correlation between the consumption of n-3 and n-6 polyunsaturated fatty acids in the diet and also their relative proportion in the diet as a prevention or stopping the progression of cardiovascular disease. Furthermore, in this thesis I have elucidated the causes of cardiovascular diseases, I have become familiar with the pathophysiology of these diseases, I have elucidated the function of polyunsaturated fatty acids (α-linolenic acid and its metabolites and linoleic acid and its resulting metabolites) in the human body and their metabolism. Finally, I made dietary recommendations resulting from lifestyle and dietary patterns as well as elucidate risk factors associated with the development of cardiovascular diseases.
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Biotechnological potential of algae and cyanobacteria from low temperature environments
Faško, Adam ; Nedbalová, Linda (advisor) ; Vítová, Milada (referee)
With the interest in biofuels rising, public awareness of the biotechnological potential of algae and cyanobacteria has grown and there is discussion about other possible uses of these microorganisms. Algae and cyanobacteria from low temperature environments possess exceptional properties as part of their adaptation strategies. The aim of this work is to evaluate whether these properties can be exploited in biotechnology. In my theses, I focus on the three most frequently discussed areas of application of algae and cyanobacteria from cold environments: production of polyunsaturated fatty acids, dyes and antioxidants, and wastewater treatment. Based on the available literature, I concluded that algae and cyanobacteria from low temperature environments can outperform mesophilic species in terms of production of desired substances or nutrient uptake from the medium under certain conditions. Selection of suitable strains, optimization of culture conditions and application of metabolic engineering and synthetic biology will have a major impact on the utilization rate of these microorganisms.
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Consumption and species representation of vegetable oils in the daily diet families in the Czech Republic
MARHOUNOVÁ, Milada
The bachelor thesis focuses on consumption and species representation of vegetable oils, fats in the daily diet families in the Czech households. In the theoretical section, I conducted a literary research of available resources regarding to the subject. This section focuses on definition of oils and fats, their chemical composition fats and fatty acids. Well-known and less known vegetable oils with the highest contents of saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids, omega -6 and omega -3 fatty acids. Also processing and use of vegetable oils. Importance of fats in human nutrition and nutritional recomendations. In the practical section is described methodology of the research. I chose a questionnaire method to collect the datas. Questionnaire survey focuses on consumption and species representation of vegetable oils, fats in the daily diet families in the Czech households. What are the criteria for shopping of vegetable oils and their usage in the kitchens. Whether people know about positive effescts of omega- 6 and omega -3 fatty acids in human nutrition and also importance to human health. The partial results are characterised and discussed in the conclusion of this work.
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Determination of fatty acids in human tissues
Turňová, Ivana ; Kuchařová, Monika (advisor) ; Nováková, Veronika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Ivana Turňová Supervisor of Diploma Thesis: Mgr. Monika Kuchařová, Ph.D. Consultant: prof. MUDr. Zdeněk Zadák, CSc. Title of Diploma Thesis: Determination of fatty acids in human tissues The fatty acids are non-negligible component of lipids as one of the basic nutrients. This thesis in its theoretical part presents above all the group of polyunsaturated fatty acids (PUFA), which are important structural units of the cell membranes, they are also the precursors to several significant biologically active substances. In the human organism PUFA participate in many physiological and pathological processes this way, where they cause the large spectrum of actions. Onwards the thesis describes the gas chromatography method that is used in chemical analysis of lipids for the fatty acid determination as the gold standard. Experimentally, the fatty acid representation was determined in the blood, muscle, heart, liver and kidney among 26 cadavers divided into two groups according to the presence/absence of inflammatory process at the time of death. The data obtained were statistically analysed. The results were descriptively evaluated and there were indicated possible explanations of differences...
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The Potential Use of the Eustigmatophyceae In the Production of Biofuels
Pilátová, Jana ; Němcová, Yvonne (advisor) ; Pichrtová, Martina (referee)
The eustigmatophytes - yellow-green microalgae - are well known for their high lipid content which is constituted by high amounts of essential polyunsaturated fatty acids (PUFA), such as eicosapentaenoic acid (EPA). This, combined with their high growth rate and an exceptionally high adaptability, makes them very promising feedstock for the biofuels production. In this bachelor thesis the role of eustigmatophytes in the production of biofuels is depicted with consideration of the issue of various cultivation conditions effects on the lipid content and composition. The first review of fatty acid distribution in eustigmatophytes ever reported is provided herein. Thus this work might be tentatively contributing to the strain selection issue - one of the most discussed topics in the biofuels field. The fatty acid distribution is qualitatively and quantitatively changed under various chemical and physical stress conditions. Thus the impact of nitrogen and phosphorous availability alongside with light intensity or temperature on lipid changes are presented. In addition, the process of fatty acid and polyunsaturated fatty acid synthesis is briefly outlined. Recent genetic engineering trends and successes are also mentioned, such as the implementation of high-efficiency homologous recombination to...
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Determination of the fatty acids spectrum in patients undergoing treatment with taxanes
Kuříková, Barbora ; Kuchařová, Monika (advisor) ; Nováková, Veronika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Author of Diploma Thesis: Bc. Barbora Kuříková Supervisor of Diploma Thesis: Mgr. Monika Kuchařová, Ph.D. Consultant: prof. MUDr. Zdeněk Zadák, CSc. Title of Diploma Thesis: Determination of the fatty acids spectrum in patients undergoing treatment with taxanes The diploma thesis deals with the determination of fatty acids spectrum in erythrocytes in patients treated with taxanes. The theoretical part describes general features of fatty acids, their synthesis and degradation. Then it is focused on the topic of breast cancer, taxane treatment and negative side effects associated with taxane treatment, especially polyneuropathy. The gas chromatography, which is commonly used in practice, is also described in this part. The experimental part is divided into three parts. The first part describes working process, chromatographic analysis and evaluation of this analysis. In the second part there is comparing of spectrum of fatty acids of patients without and with polyneroupathy. And in the last part there is a comparing of spectrum of fatty acids of patients before taxane treatment, closely after treatment and about month after the treatment. Results of the experimental part are evaluated at the end...
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Diversity, ecology and ecophysiology of snow algae
Mikešová, Lenka ; Nedbalová, Linda (advisor) ; Kvíderová, Jana (referee)
Permanent and temporary snow cover in polar and mountain areas is a very extreme habitat. However, there are organisms that inhabit this environment. Green algae of the order Chlamydomonadales (Chlorophyta) belong among the important representatives of cryoseston, which are known from polar and mountain regions around the world. These organisms, which occupied the snow environment probably secondarily, developed different ecophysiological adaptations required for the successful survival in extreme conditions. Low temperatures and high levels of radiation are the main extreme factors that organisms must cope with. The most important survival mechanisms include the adaptation of life cycles (such as of the shifts between resistant stages and moving vegetative stages), accumulation of secondary carotenoids, changes in fatty acid composition of membranes and lowering of growth temperature optima in some species. These adaptations together with the study of diversity represent at present the main subjects of research of snow algae.
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Study of metabolic syndrome in mice model: roles of dietary lipids, adipose tissue and AMP-activated protein kinase
Medříková, Daša ; Kopecký, Jan (advisor) ; Houštěk, Josef (referee) ; Novotný, Jiří (referee)
Obesity and associated metabolic disorders, e. g. metabolic syndrome, represent a considerable health threat for modern society. Due to sedentary lifestyle, high caloric intake and changes in composition of diet, prevalence of obesity is increasing worldwide. One of the possible causes contributing to higher prevalence of obesity in recent population could be the change of fatty acids (FA) composition of dietary lipids, with the shift in the content of n-6 and n-3 FA toward n-6 FA. In contrast to n-6 FA, n-3 FA are known for their anti-atherogenic, anti-obesogenic and anti-inflammatory properties. In our experiments in mice, the capability of naturally occurred and chemically modified n- 3 long chain polyunsaturated fatty acids (LC-PUFA) in prevention and reversal of specific parts of metabolic syndrome was demonstrated. A specific chemical derivative of docosahexaenoic acid was proven to be very effective in preventing and improving metabolic conditions of animals exposed to high-fat (HF) diet challenge. Further, the involvement of AMP-activated protein kinase (AMPK), a master regulator of lipid metabolism, in skeletal muscle thermogenesis induced by HF-feeding was investigated. Activation of AMPK in the HF-fed mice is most possibly caused by increased leptin levels and represents an important link...
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The fluctuation of fatty acids levels in the tissues of suddenly deceased persons and comparison with values in pathological states
Čunátová, Alena ; Kuchařová, Monika (advisor) ; Lázníčková, Alice (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Alena Čunátová Supervisor of Diploma Thesis: Mgr. Monika Kuchařová, Ph.D. Title of Diploma Thesis: The fluctuation of fatty acids levels in the tissues of suddenly deceased persons and comparison with values in pathological states Fatty acids and their metabolites are significantly involved in many physiological and pathological processes. This thesis monitors the levels of selected fatty acids in human tissues and the effect of long-term disease on their stores. In addition to the general characteristics, the theoretical part focuses on the intake of polyunsaturated fatty acids and their importance in the human body. It also deals with the effects of eicosanoids and other fatty acid metabolites. Methods used in fatty acid bioanalysis are also described. In the experimental part, the proportion of fatty acids in seven tissue types was compared between two groups of donors. One of them included 8 relatively young, healthy, suddenly deceased individuals. The second group included 12 elderly polymorbid patients. The obtained tissue samples were adjusted using extraction and derivatization techniques. Gas chromatography with flame ionization detection was used for analysis. The measured...
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The Importance of Nutrition, The Quality and Effective Doses of Food Suplements EPA/DHA
Krčmářová, Miluše ; Pejšová, Hana (advisor) ; Chrpová, Diana (referee)
The fats should be understood as a necessary part of a diet and their income should be assessed from the energetic point of view, as well as fats to other nutrients ratio and the kind of fatty acids contained. After a period of total refusal of fats consumption as the cause of obesity and civilization disease occurences, the opinion, that it's not good to remove them from the diet because has prevailed. The reason is there might arise a deficiency in unsaturated essential acids, which are necessary to secure many functionalities in the organism. Among the interest became the polyunsaturated fatty acids with their low presence in the central european diet. This is related to the topic of dietary supplements with omega-3 fatty acids, which is the main topic of this thesis. The goal of this bachelor thesis is to sum up the importance of food supplemens with omega-3 fatty acids usage, to compare quality of the most common products and to bring information about their effective recommended doses. The main focus has been finding reasons why clients of a chosen pharmacy are looking for these products, whether there is a dirtect connection between their health difficulties a their selection of concrete product and what influences their choice. Further, the level of their knowledge about the quality of...
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